NAD+ (Nicotinamide Adenine Dinucleotide) is a naturally occurring coenzyme found in every living cell. It plays a central role in cellular energy metabolism, DNA repair, and the regulation of biological aging processes.
Mechanism of action
Serves as an essential electron carrier in cellular respiration — shuttling electrons during glycolysis, the citric acid cycle, and oxidative phosphorylation to generate ATP. Also activates sirtuins (longevity proteins), PARP enzymes for DNA repair, and CD38/cyclic ADP-ribose signaling pathways.
Research context
Research has extensively studied NAD+ in the context of aging, mitochondrial dysfunction, neurodegeneration, and metabolic health. Declining NAD+ levels with age correlate with reduced energy production and impaired cellular repair capacity.
⚠ For research purposes only. Not approved for human use by the FDA. Not for human consumption.
Research dosing reference
Starting dose
250–500 mg
Frequency
Daily or every other day
Max studied
1000 mg / day
Route
IV or Subcutaneous
Protocol notes
250–500 mg administered IV or subcutaneously. IV produces faster effects. SubQ is more practical for ongoing protocols. Start lower to assess tolerance.
Commonly observed side effects
IV administration may cause flushing, nausea, or chest tightness if infused too quickly — slow the infusion rate. SubQ is generally well tolerated.
⚠ Dosing data sourced from published clinical literature. For research reference only.
Reconstitution protocol
01
Draw 5–10 mL of bacteriostatic water or sterile saline into a clean syringe.
02
Insert needle through stopper angled toward the glass wall.
03
Slowly push the solvent in along the wall of the vial.
04
Swirl gently until fully dissolved. NAD+ dissolves quickly — solution should be clear to pale yellow.
05
Label with reconstitution date and use promptly or refrigerate.
Bacteriostatic water volume
5–10 mL bacteriostatic water or sterile saline per 500 mg vial
Dose calculation referenceAt 500 mg in 10 mL: each 1 mL = 50 mg · each 5 mL = 250 mg
⚠ Always use bacteriostatic water for multi-dose vials. Never shake — always swirl gently.
Dose Calculator
Enter how much bacteriostatic water you added to your 500mg vial and your target dose. The calculator shows exactly how many units to draw on a standard U-100 insulin syringe.
500 mg
Draw to this mark on your syringe
— units
— mL
Concentration: — mg/mL
⚠ For research reference only. Calculations assume a standard U-100 insulin syringe (100 units = 1 mL). Always verify your measurements.
Injection protocol
01
Draw calculated dose into appropriate syringe or IV bag.
02
For SubQ: clean site, pinch skin, insert at 45°, slowly inject.
03
For IV: administer slowly over 1–4 hours. Never push rapidly.
Log time, dose, route, and any observed reactions.
Recommended needle
25–27 gauge for SubQ · IV line for infusion
Injection sites
Subcutaneous: abdomen or outer thigh. IV: standard peripheral line.
⚠ Always rotate injection sites. Never reuse syringes. Dispose of sharps properly.
Storage conditions
Unreconstituted
2–8°C refrigerated · Up to 24 months — protect from light
Reconstituted
2–8°C refrigerated · Use within 24–48 hours
Stability notes
NAD+ is light-sensitive. Store in amber vials or away from direct light. Reconstituted solution should be used promptly — stability decreases rapidly after reconstitution. Do not freeze.
⚠ Keep away from children. Do not use if solution appears cloudy or contains particulates.
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